The hippocampus, anterior thalamic nuclei, and retrosplenial cortex (RSC) are well-established hubs within the spatial memory network. However, the precise contribution of the RSC in episodic memory is unknown. This project aims to investigate the role of the RSC in spatial memory by utilizing reversible pharmacological inactivation. A cohort of Long-Evans rats was trained on a spatial navigation task to locate three baited wells on a circular arena. Asymptotic performance was defined as 70% success within a single session, where the rat obtained all baits utilizing a consistent path. They underwent testing across three distinct reward-well configurations. Rats achieved asymptotic performance in the first well configuration before receiving an intracranial saline injection. This injection, serving as an internal control, was followed by a session where they were tested on both the learned well configuration and a novel set of wells. After this test session, animals were trained until asymptotic performance before receiving another intracranial injection of muscimol, a GABAA agonist (Allen et al., 2008). Rats were then tested on 10 trials of recall, followed by 10 trials of a third novel configuration within a single session. Finally, an afternoon comparative control session was run to isolate the RSC’s involvement in memory encoding. We hypothesize that RSC inactivation will impair both the acquisition and recall of novel spatial configurations. Ultimately, this work yields new insight into RSC’s involvement in spatial episodic memory.